Publications
2009
Timmins NE, Palfreyman E, Marturana F, Dietmair S, Luikenga S, Lopez G, Fung YL, Minchinton R, Nielsen LK (2009) Clinical Scale Ex vivo Manufacture of Neutrophils from Hematopoietic Progenitor Cells. Biotechnol Bioeng., accepted 2.6.2009.
Joachimsthal EL, Reeves RKH, Hung J, Nielsen LK, Ouwerkerk D; Klieve AV; Vickers CE (2009) Production of bacteriocins by Streptococcus bovis strains from Australian ruminants. Journal of Applied Microbiology, accepted 2.6.2009.
Chen W, Marcellin E, Nielsen LK (2009) Hyaluronan molecular weight is controlled by UDP-N- acetylglucosamine concentration in Streptococcus zooepidemicus. J Biol Chem, accepted 16/5/2009.
Timmins NE, Nielsen LK (2009) Blood cell manufacture: current methods and future challenges. TiBTech, accepted.
Quek L-E, Wittmann C, Nielsen LK, Krömer JO (2009) OpenFLUX: Efficient Modelling Software for 13C-Based Metabolic Flux Analysis. Microbial Cell Factories 8:25.
Marcellin E, Gruber CW, Archer C, Craik DJ, Nielsen LK (2009) Proteome analysis of the hyaluronic acid-producing bacterium, Streptococcus zooepidemicus. Proteome Science 7:13. doi:10.1186/1477-5956-7-13
Marcellin E, Chen W, Nielsen LK (2009) Microbial hyaluronic acid biosynthesis. In: Microbial Production of Biopolymers and Polymer Precursors: Applications and Perspectives, ed. BHA Rehm. Norwich: Caister Academic Press. Pp. 163-180.
Marcellin E, Nielsen LK, Abeydeera P, Krömer JO (2009) Quantitative analysis of intracellular sugar phosphates and sugar nucleotides in encapsulated Streptococci using HPAEC-PAD. Biotechnol. J. 4: 58–63.
2008
Quek L-E, Nielsen LK (2008) On the reconstruction of the Mus musculus genome-scale metabolic network model. Genome Informatics 21:89-100.
Duke M, Lim A, da Luz S, Nielsen L (2008) Lactic acid enrichment with inorganic nanofiltration and molecular sieving membranes by pervaporation. Food and Bioproducts Processing 86:290-295.
Renouf MA, Wegener MK, Nielsen LK (2008) An environmental life cycle assessment comparing Australian sugarcane with US corn and UK sugar beet as producers of sugars for fermentation. Biomass & Bioenergy 32: 1144 – 1155. doi:10.1016/j.biombioe.2008.02.012
Leung A, Trau M, Nielsen LK (2008) Assembly of multilayer PSS/PAH membrane on coherent alginate/PLO microcapsule for long-term graft transplantation. Journal of Biomedical Materials Research: Part A 88A: 226-237.
Lee SY, Park JH, Jang SH, Nielsen LK, Kim J, Jung KS (2008) Fermentative butanol production by Clostridia. Biotechnology and Bioengineering 101: 209-228.
Blank LM, Hugenholtz P, Nielsen LK (2008) Evolution of the hyaluronic acid synthesis (has) operon in Streptococcus zooepidemicus and other pathogenic streptococci. Journal of Molecular Evolution 67: 13-22.
Leung A, Lawrie G, Nielsen LK, Trau M (2008) Facilitating Specific Functionality on the APA Microcapsule to Control Its Neighborhood: Application in Implementing Local Immunosuppression for Xenotransplanation. Journal of Microencapsulation 25: 387-398.
Song H, Kim TY, Choi B-K, Choi SJ, Nielsen L, Chang HN, Lee SY (2008) Development of chemically defined medium for Mannheimia succiniciproducens based on its genome sequence. Appl. Microbiol. Biotechnol.79: 263-272. DOI 10.1007/s00253-008-1425-2
Gnanasambandam A, Anderson DJ, Purnell M, Nielsen LK, Brumbley SM (2008)The N-terminal presequence from F1-ATPase -subunit of Nicotiana plumbaginifolia efficiently targets green fluorescent fusion protein to the mitochondria in diverse commercial crops. Functional Plant Biology, 35: 166-170.
Hines M, Nielsen LK, Cooper-White J (2008) The hematopoietic stem cell niche: what are we trying to replicate? Journal of Chemical Technology and Biotechnology 83:421-443. doi: 10.1002/jctb.1856
Koebmann BJ, Blank LM, Solem C, Petranovic D, Nielsen LK, Jensen PR (2008) Increased biomass yield of Lactococcus lactis during energetically limited growth and respiratory conditions. Biotechnol Appl Biochem. 50:25-33. doi:10.1042/BA20070132
2007
Timmins NE, Nielsen LK (2007) Generation of Multicellular Tumour Spheroids by the Hanging Drop Method. In: Tissue Engineering 2nd Edition, eds. Fussenegger M, Hauser H. Methods in Molecular Medicine 140: 141-151.
Brumbley SM, Purnell MP, Petrasovits LA, Nielsen LK, Twine PH (2007) Developing the sugarcane biofactory for high-value biomaterials. Int Sugar J 109: 5-15.
Purnell MP, Petrasovits LA, Nielsen LK, Brumbley SM (2007) Spatio-temporal characterisation of polyhydroxybutyrate accumulation in sugarcane. Plant Biotechnology Journal 5:173-184. doi: 10.1111/j.1467-7652.2006.00230.x
Petrasovits LA, Purnell MP, Nielsen LK, Brumbley SM (2007) Production of polyhydroxybutyrate in sugarcane. Plant Biotechnology Journal 5:162-172. doi: 10.1111/j.1467-7652.2006.00229.x
2006
Joachimsthal EL, Hung J, Nielsen LK (2006) Lactic acid production by a rumen bacterium from sugarcane processing co-product streams. Proceedings of ASSCT.
Peng JC, Hyde C, Pai S, O’Sullivan BJ, Nielsen LK, Thomas R (2006) Monocyte-derived DC primed with TLR agonists secrete IL-12p70 in a CD40-dependent manner under hyperthermic conditions. Journal of Immunotherapy 29:606-615.
Pedrini MRD, Chan LCL, Nielsen LK, Reid S, (2006) In vitro production of Helicoverpa armigera single-nucleocapsid nucleopolyhedrovirus. Brazilian Archives of Biology and Technology 49: 35-41SI.
Pedrini MRD, Christian P, Nielsen LK, Reid S, Chan LCL (2006) Importance of virus-medium interactions on the biological activity of wild-type Heliothine nucleopolyhedroviruses propagated via suspension insect cell cultures. Journal of Virological Methods 136:267-272.
Dinnis DM, Stansfield SH, Schlatter S, Smales CM, Alete D, Birch JR, Racher AJ, Marshall CT, Nielsen LK, James DC (2006) Functional proteomic analysis of GS-NS0 murine myeloma cell lines with varying recombinant monoclonal antibody production rate. Biotechnology and Bioengineering 94: 830-841. DOI 10.1002/bit. 20899
Peng JC, Abu Bakar S, Richardson MM, Jonsson JJ, Frazer IH, Nielsen LK, Morahan G, Thomas R (2006) IL10 and IL12B polymorphisms each influence IL-12p70 secretion by dendritic cells in response to LPS. Immunology and Cell Biology 84: 227-232. doi:10.1111/j.1440-1711.2006.01419.x
Brumbley SM, Twine PE, Nielsen LK (2006) Plastics from Plant Cells: The High-Carb Economy. Australasian Science 27:19-22.
2005
Pedrini MRS, Nielsen LK, Reid S, Chan LCL (2005) Properties of a unique mutant of Helicoverpa armigera single-nucleocapsid nucleopolyhedrovirus that exhibits both Many Polyhedra and Few Polyhedra phenotypes upon extended serial passaging in cell cultures. In Vitro Cellular & Developmental Biology – Animal 41:289-297.
Peng JC, Thomas R, Nielsen LK (2005) Generation and maturation of DC for clinical application under serum-free conditions. Journal of Immunotherapy 28:599-609.
Leung A, Ramaswamy Y, Munro P, Lawrie G, Nielsen LK, Trau M (2005) Emulsion Strategies in the Microencapsulation of Cells: Pathways to Thin Coherent Membranes. Bioengineering & Biotechnology 92:45-53.
Haas R, Nielsen LK (2005) A Physiological Product-Release Model for Baculovirus Infected Insect Cells. Bioengineering & Biotechnology 91: 768-772.
Timmins NE, Maguire TL, Grimmond SM, Nielsen LK (2005). Identification of three gene candidates for multicellular resistance in colon carcinoma. Cytotechnology 46:9-18. http://dx.doi.org/10.1007/s10616-005-1476-5.
Blank LM, McLaughlin RL, Nielsen LK (2005) Stable production of hyaluronic acid in Streptoccus zooepidemicus chemostats operated at high dilution rate. Biotechnology & Bioengineering 90: 685-693.
Timmins NE, Harding FJ, Smart C, Brown MA, Nielsen LK (2005) Method for the generation and cultivation of functional 3-dimensional mammary constructs without exogenous extracellular matrix material. Cell & Tissue Research 320:207-210. DOI: 10.1007/s00441-004-1064-6
Sheikh K, Förster J, Nielsen LK (2005) Modeling Hybridoma Cell Metabolism Using a Generic Genome-Scale Metabolic Model of Mus musculus. Biotechnology Progress 21:112-121. DOI: 10.1021/bp0498138
Fong Chong B, Blank LM, McLaughlin R, Nielsen LK (2005) Microbial hyaluronic acid production. Applied Microbiology and Biotechnology 66:341-351. DOI: 10.1007/s00253-004-1774-4.